张剑

副教授，硕士生导师

E-Mail：zhj8226@sdu.edu.cn

教育背景

博士 2005.9–2010.6 华中农业大学  动物遗传育种与繁殖

学士 1998.9–2002.6 华中农业大学  畜牧

工作经历

2020.9-至今，57365线路检测中心官网，yl12311线路检测，副教授

2012.6-2020.8，57365线路检测中心官网，yl12311线路检测，讲师

2010.6-2012.6，57365线路检测中心官网，yl12311线路检测，博士后

科研方向

1. 利用动物模型开展人类疾病的致病分子机理研究

2. 基因突变与听觉发育、生殖等相关性研究

3. 细胞恶性转化与肿瘤

主持课题

1. 国家重点研发计划，抗生素耐药因子的来源、动员和扩散-3，2022-10至 2025-09，主持

2. 国家自然科学基金委员会，重大项目，老年性聋的机制和干预研究，2022-01-01 至 2026-12-31，课题骨干

3. 国家重点研发计划，精子成熟关键基因突变动物模型的构建与功能研究-3，2018-12至2021-12，主持

4. 山东省自然科学基金委员会，面上项目，LOXL3突变导致人Stickler综合征的分子机制研究，2017-08至2020-06，主持

5. 57365线路检测中心官网，交叉学科培育项目，基于CRISPR-Cas9技术的人DFNB105耳聋疾病动物模型的制作及其分析研究，2017-01至2019-12，主持

6. 山东省自然科学基金委员会，青年项目，TPRN基因在小鼠内耳中的功能研究，2013-12至2016-12，主持

代表性论文

1. Ziyi Liu^(#), Fan Mo^(#), Xinyu Dong, Ge Chen, Jiangang Gao^(*), Jian Zhang^(*). Progressive degeneration of the retina in Loxl3 mutant mouse model of Stickler syndrome. Developmental Biology, 2023, 495:54-62.

2. Xin Wei^(#), Jian Zhang^(#), Bianfang Wang, Wenjia Wang, Yuqing Sun, Ling Li, Hai Xu(*), Mingyu Wang(*).Spatially and Temporally Confined Response of Gastrointestinal Antibiotic Resistance Gene Levels to Sulfadiazine and Extracellular Antibiotic Resistance Gene Exposure in Mice. Biology (Basel), 2023, 12(2):210.

3. Ziyi Liu, Xinfeng Bai, Peifeng Wan, Fan Mo, Ge Chen, Jian Zhang^(*) , Jiangang Gao^(*), Targeted Deletion of Loxl3 by Col2a1-Cre Leads to Progressive Hearing Loss. Frontiers in Cell and Developmental Biology, 2021, 9:683495.

4. Zongzhuang Wen^(#), Haixia Zhu, Aizhen Zhang, Jing Lin, Guangkai Zhang, Dongyue Liu, Yu Xiao, Chao Ye, Daqing Sun^(*), Bin Wu ^(*), Jian Zhang^(*), Jiangang Gao^(*)，Cdc14a has a role in spermatogenesis, sperm maturation and male fertility, Experimental Cell Research, 2020, 395 (1): 112178.

5. Hui Xiao^(#), Ning Ding, Hang Liao, Zhigang Yao, Xiankui Cheng, Jian Zhang^(*), Miaoqing Zhao^(*), Prediction of relapse and prognosis by expression levels of long noncoding RNA PEG10 in glioma patients. Medicine, 2019, 98(45):e17583.

6. Zongzhuang Wen^(#), Haixia Zhu^(#), Zhenzu Li^(#), Sen Zhang, Aizhen Zhang, Tingting Zhang, Xiaolong Fu, Daqing Sun^(*), Jian Zhang^(*), Jiangang Gao^(*)，A knock-in mouse model of Pendred syndrome with Slc26a4 L236P mutation，Biochemical and Biophysical Research Communications, 2019, 515 (2):359-365.

7. Men, Yuqin^（#）; Zhang, Aizhen; Zhang, Liwen; Jin, Yecheng; Wang, Zhishuo; Zhao, Jing; Yu, Xiaolin; Zhang, Jian^（*）; Gao, Jiangang^（*）, Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice., Frontiers of Medicine, 2016, 10(4), pp 481-489.

8. Zhang, Jian^（#）; Liu, Ziyi; Chang, Aoshuang; Fang, Jie; Men, Yuqin; Tian, Yong; Ouyang, Xiaomei; Yan, Denise; Zhang, Aizhen; Sun, Xiaoyang; Tang, Jie; Liu, Xuezhong^（*）; Zuo, Jian^（*）; Gao, Jiangang^（*）, Abnormal mRNA splicing but normal auditory brainstem response (ABR) in mice with the prestin (SLC26A5) IVS2-2A > G mutation, Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis,2016, 790, pp 1-7.

9. Zhang, Jian^（#）; Liu, Ziyi; Zhang, Tingting; Lin, Zhuchun; Li, Zhenzu; Zhang, Aizhen; Sun, Xiaoyang; Gao, Jiangang^（*）, Loss of Lysyl Oxidase-like 3 Attenuates Embryonic Lung Development in Mice, Scientific Reports,2016, 6, pp 33856.

10. Zhang, Jian^（#）; Yang, Rui; Liu, Ziyi; Hou, Congzhe; Zong, Wen; Zhang, Aizhen; Sun, Xiaoyang; Gao, Jiangang^（*）, Loss of lysyl oxidase-like 3 causes cleft palate and spinal deformity in mice., Human Molecular Genetics, 2015,24(21), pp 6174-6185.

11. Zong, Wen^（#）; Jiang, Yan; Zhao, Jing^（*）; Zhang, Jian^（*）; Gao, Jian-Gang, Estradiol plays a role in regulating the expression of lysyl oxidase family genes in mouse urogenital tissues and human Ishikawa cells., J Zhejiang Univ Sci B, 2015, 16(10), pp 857-864.

12. Zhang, Jian^（#）; Zhao, Jing^（#）; Jiang, Wen-jie; Shan, Xi-wei; Yang, Xiao-mei^（*）; Gao, Jian-gang^（*）, Conditional gene manipulation: Cre-ating a new biological era., J Zhejiang Univ Sci B, 13(7), 2012, pp 511-524.

13. Men, Yuqin^（#）; Zhang, Aizhen; Li, Haixiang; Zhang, Tingting; Jin, Yecheng; Li, Huashun; Zhang, Jian^（*）; Gao, Jiangang^（*）, LKB1 Is Required for the Development and Maintenance of Stereocilia in Inner Ear Hair Cells in Mice.,2015, PLos One, 10(8): e0135841-e0135841.

14. 张剑^（#）; 杨晓梅^（*）; 高建刚^（*）, 小鼠基因敲除的研究进展, 57365线路检测中心官网学报(理学版), 2011，(10), pp 183-196。

专利

张剑^(#); 高建刚; 侯丛哲; 杨瑞; 刘子熠,一种小鼠Loxl3基因条件性敲除的方法与应用, 2017.6.6-2037.6.5, 中国, 201510080652 .5.